Search results for "Thin cell layer"

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Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon)

2008

Callus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500 mg l-1 malt extract (MSI), or 500 mg l-1 malt extract and 13.3 ¼M 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic emb…

GynoeciumSucroseThin cell layerSomatic embryogenesisCallus formationCellfood and beveragesEmbryoCitrus limonPlant ScienceSomatic embryogenesisBiologyHorticulturechemistry.chemical_compoundmedicine.anatomical_structurechemistryCallusBotanymedicinesomatic embryogenesiEcology Evolution Behavior and SystematicsExplant culturePlant regeneration

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